Biochemical Assay - Safety Panel Assays

Reaction BiologyおよびEuroscrrenFAST (former Euroscreen)では、GPCR、イオンチャネル、トランスポーター、CYP等、ヒトにおいて薬物有害反応を引き起こす可能性のある幅広いターゲットに対する、化合物のオフターゲットスクリーニングに有用なパネルアッセイを提供しています。新薬開発の早い段階での安全性評価等にご利用いただけます。

• Euroscreen Safety Panel (45ターゲット)
• Reaction Biology In Vitro Evaluation of Safety and Toxicity (InVEST) panel (53ターゲット)

Safety panel (Euroscreen)

45種のGPCR、トランスポーター、イオンチャネル、各種酵素のパネルです。

	Target family	Target name	Species / Cell Line	Assay / Tracer
1	GPCR	Adenosine A2A	Human	cAMP
2	GPCR	Adrenergic alpha1a	Human	cAMP
3	GPCR	Adrenergic alpha2a	Human	cAMP
4	GPCR	Adrenergic beta2	Human	cAMP
5	GPCR	Cannabinoid CB1	Human	GTP
6	GPCR	Cholecystokinin CCK1	Human	AEQ
7	GPCR	Dopamine D1	Human	cAMP
8	GPCR	Dopamine D2S	Human	cAMP
9	GPCR	Endothelin ETA	Human	AEQ
10	GPCR	Endothelin ETB	Human	AEQ
11	GPCR	Histamine H1	Human	AEQ
12	GPCR	Histamine H2	Human	cAMP
13	GPCR	Melatonin MT1	Human	GTP
14	GPCR	Melatonin MT2	Human	GTP
15	GPCR	Muscarinic M1	Human	AEQ
16	GPCR	Muscarinic M2	Human	cAMP
17	GPCR	Opioid delta	Human	cAMP
18	GPCR	Opioid kappa	Human	cAMP
19	GPCR	Opioid mu	Human	cAMP
20	GPCR	Serotonine 5-HT1A	Human	cAMP
21	GPCR	Serotonine 5-HT1B	Human	cAMP
22	GPCR	Serotonine 5-HT2A	Human	IPOne
23	GPCR	Serotonine 5-HT2B	Human	IPOne
24	GPCR	Serotonine 5-HT3	Human	AEQ
25	GPCR	Vasopressin V1a	Human	AEQ
26	Acetylcholin esterase	AChE	Electric Eel	Fluorescence
27	Cyclooxygenase	COX-2	Human	Colorimetric
28	Monoamine Oxidase	MAO-A	Human	Luminescence
29	Phosphodiesterase	PDE-3	Human	HTRF
30	Phosphodiesterase	PDE-4	Human	HTRF
31	Cytochrome P450	1A2	Human	Luminescence
32	Cytochrome P450	2C9	Human	Luminescence
33	Cytochrome P450	2D6	Human	Luminescence
34	Cytochrome P450	2E1	Human	Luminescence
35	Cytochrome P450	3A4	Human	Luminescence
36	Amine Transporter	DAT	CHO-K1	[3H]-WIN 35,428
37	Amine Transporter	NET	HEK293	[3H]-Nisoxetine
38	Amine Transporter	SERT	U2OS	[3H]-Citalopram
39	Ion Channel	Ca2+ channel L, DHP site	rat brain	[3H]-Nitrendipine
40	Ion Channel	Ca2+ channel L, verapamil site	rat brain	[N-methyl-3H]-Desmethoxyverapamil
41	Ion Channel	hERG	HEK293	[3H]-Dofetilide
42	Ion Channel	GABA BZD (central)	rat brain	[3H]-Flunitrazepam
43	Ion Channel	NMDA (Glutamate) GRIN 1	HEK293	[3H]-MDL 105,519
44	Ion Channel	Nicotinic alpha4beta2	rat brain	[3H]-Cytisine
45	Sigma	Sigma	MCF-7	[3H]-Pentazocine

In Vitro Evaluation of Safety and Toxicity (InVEST) panel

53種のGPCR、トランスポーター、イオンチャネル、CYP450、各種酵素等のパネルです。

	Target family	Target name	Species	Assay format
1	Biogenic Amine Transporter	SET (Serotonin Transporter)	Human	Radioligand Filter Binding
2	Biogenic Amine Transporter	DAT (sodium-dependent dopamine transporter)	Human	Radioligand Filter Binding
3	Biogenic Amine Transporter	NET (norepinephrine transporter)	Human	Radioligand Filter Binding
4	Cholinesterase	Acetylcholinesterase	Human	Enzymatic Activity
5	Cyclooxygenase	COX-1	Ovine	Enzymatic Activity
6	Cyclooxygenase	COX-2	Human	Enzymatic Activity
7	Cytochrome P450	CYP1A2	Human	Enzymatic Activity
8	Cytochrome P450	CYP2A6	Human	Enzymatic Activity
9	Cytochrome P450	CYP2B6	Human	Enzymatic Activity
10	Cytochrome P450	CYP2C19	Human	Enzymatic Activity
11	Cytochrome P450	CYP2C8	Human	Enzymatic Activity
12	Cytochrome P450	CYP2C9	Human	Enzymatic Activity
13	Cytochrome P450	CYP2D6	Human	Enzymatic Activity
14	Cytochrome P450	CYP2E1	Human	Enzymatic Activity
15	Cytochrome P450	CYP2J2	Human	Enzymatic Activity
16	Cytochrome P450	CYP3A4	Human	Enzymatic Activity
17	Cytochrome P450	CYP3A5	Human	Enzymatic Activity
18	GPCR	Adenosine A1 Receptor	Human	Radioligand Filter Binding
19	GPCR	Adrenergic Alpha 1A Receptor	Human	Radioligand Filter Binding
20	GPCR	Adrenergic Alpha 2A Receptor	Human	Radioligand Filter Binding
21	GPCR	Adrenergic Beta 1	Human	Radioligand Filter Binding
22	GPCR	Adrenergic Beta 2	Human	Radioligand Filter Binding
23	GPCR	CCK1 Receptor	Human	Radioligand Filter Binding
24	GPCR	Dopamine D1 Receptor	Human	Radioligand Filter Binding
25	GPCR	Dopamine D2S Receptor	Human	Radioligand Filter Binding
26	GPCR	Dopamine D3 Receptor	Human	Radioligand Filter Binding
27	GPCR	Histamine H1 Receptor	Human	Radioligand Filter Binding
28	GPCR	Muscarinic M1 Receptor	Human	Radioligand Filter Binding
29	GPCR	Muscarinic M2 Receptor	Human	Radioligand Filter Binding
30	GPCR	Muscarinic M3 Receptor	Human	Radioligand Filter Binding
31	GPCR	Muscarinic M4 Receptor	Human	Radioligand Filter Binding
32	GPCR	Muscarinic M5 Receptor	Human	Radioligand Filter Binding
33	GPCR	Opioid delta Receptor	Human	Radioligand Filter Binding
34	GPCR	Opioid kappa Receptor	Human	Radioligand Filter Binding
35	GPCR	Opioid mu Receptor	Human	Radioligand Filter Binding
36	GPCR	Serotonin 5-HT1A Receptor	Human	Radioligand Filter Binding
37	GPCR	Serotonin 5-HT1B Receptor	Human	Radioligand Filter Binding
38	Ion Channel	hERG	Human	Fluorescence Polarization
39	Ion Channel	Central BDZ (GABA-A Receptor)	Rat	Radioligand Filter Binding
40	Ion Channel	5-HT3	Human	Radioligand Filter Binding
41	Monoamine oxidase	MAO-A	Human	Enzymatic Activity
42	Monoamine oxidase	MAO-B	Human	Enzymatic Activity
43	Nuclear Receptor	Estrogen Receptor a	Human	Fluorescence Polarization
44	Nuclear Receptor	Glucocorticoid Receptor	Human	Fluorescence Polarization
45	Nuclear Receptor	PPARg Receptor	Human	Fluorescence Polarization
46	Nuclear Receptor	Progesterone Receptor	Human	Fluorescence Polarization
47	Phosphodiesterase	PDE3A	Human	Enzymatic Activity
48	Phosphodiesterase	PDE4A	Human	Enzymatic Activity
49	Phosphodiesterase	PDE4D	Human	Enzymatic Activity
50	Phosphodiesterase	PDE4D2	Human	Enzymatic Activity
51	Protease	ACE-1	Human	Enzymatic Activity
52	Protease	Cathepsin G	Human	Enzymatic Activity
53	Protease	Thrombin Alpha	Human	Enzymatic Activity