Reaction Biologyでは、様々なターゲットに対する酵素アッセイを実施しています。下記ターゲットの他、カスタムアッセイにも対応しておりますので、ご希望のターゲットがリストにない場合は、お問い合わせください。

  • PARP Assays (3種類)
  • Protease Assays (78種類)
  • Phosphatase Assays (21種類)
  • Apoptosis Pathway Assays (6種類)
  • Metabolic Pathway Assays (18種類)
  • Phosphodiesterase (PDE) Assays (17種類)
  • Ubiquitin-Proteasome Pathway Assays (23種類)
  • Heat Shock Protein Assays (2種類)
  • Heat Shock Protein Assays (3種類)

Poly (ADP-ribose) Polymerase (PARP) Assays

NAD/NADH-Glo™によりPARP酵素活性を測定します。
Target name
1PARP1
2PARP2
3PARP5B

Protease Assays

蛍光ペプチド基質を用いて、プロテアーゼのタンパク質分解活性を測定します。
* Protease targets not part of the monthly full screening panels
Target name
1ACE1
2ACE2
3Activated Protein C
4ADAM10
5BACE 1
6Calpain 1
7Caspase 1
8Caspase 2
9Caspase 3
10Caspase 4
11Caspase 5
12Caspase 6
13Caspase 7
14Caspase 8
15Caspase 9
16Caspase 10
17Caspase 11
18Caspase 14
19Cathepsin B
20Cathepsin C
21Cathepsin D
22Cathepsin E
23Cathepsin G
24Cathepsin H
25Cathepsin K
26Cathepsin L
27Cathepsin S
28Cathepsin V
29Chymase
30Chymotrypsin
31DPP IV
32DPP VIII
33DPP IX
34Elastase
35Factor VIIa
36Factor IXa
37Factor Xa
38Factor XIa
39Factor XIIa
40Furin
41Granzyme B*
42Hepsin*
43HIV-1
44Kallikrein 1
45Kallikrein 2
46Kallikrein 5
47Kallikrein 7
48Kallikrein 12
49Kallikrein 13
50Kallikrein 14
51Matriptase 2
52MMP1
53MMP2
54MMP3
55MMP7
56MMP8
57MMP9
58MMP10
59MMP12
60MMP13
61MMP14
62Neprilysin
63Papain
64Plasma Kallikrein
65Plasmin
66Proteinase A
67Proteinase K
68SARS-CoV-2 Mpro
69SARS-CoV-2 PLpro
70TACE
71Thrombin
72TMPRSS11D
73TMPRSS2
74tPA*
75Trypsin
76Tryptase b2
77Tryptase g1
78Urokinase

Phosphatase Assays

蛍光基質を用いて、ホスファターゼ活性を測定します。
* Phosphatase targets not part of the full screening panels
Target name
1DUSP22/MKPX
2PP1A
3PP1B
4PP2A alpha/PPP2R1A Complex
5PP2C-alpha
6PP2C-gamma
7PPAC (ACP1/LMW-PTP-A)
8PPAC (ACP1/LMW-PTP-B)*
9PTEN
10PTPN1/PTP1B-CD
11PTPN1/PTP1B-FL*
12PTPN11/SHP2 (E76K)-FL
13PTPN11/SHP2-CD
14PTPN11/SHP2-FL
15PTPN12/PTP-PEST
16PTPN2/TC-PTP
17PTPN6/SHP1
18PTPN7/LC-PTP
19PTPRB
20PTPRC/CD45
21PTPRJ/CD148

Apoptosis Pathway Assays

Target nameAssay format
1Bcl-2HTRF
2BCL-xLHTRF, SPR
3cIAP-1Fluorescence Polarization
4cIAP-2Fluorescence Polarization
5MCL1HTRF
6XIAPFluorescence Polarization

Metabolic Pathway Assays

TargetTarget nameAssay format
1Isocitrate Dehydrogenase (IDH)IDH1 WTDiaphorase/resazurin-coupled
2IDH1 G97DDiaphorase/resazurin-coupled
3IDH1 R100ADiaphorase/resazurin-coupled
4IDH1 R100QDiaphorase/resazurin-coupled
5IDH1 R132CDiaphorase/resazurin-coupled
6IDH1 R132HDiaphorase/resazurin-coupled
7IDH2 WTDiaphorase/resazurin-coupled
8IDH2 Y139DDiaphorase/resazurin-coupled
9IDH2 R140KDiaphorase/resazurin-coupled
10IDH2 R140QDiaphorase/resazurin-coupled
11IDH2 R172QDiaphorase/resazurin-coupled
12CarboxylaseACC1ADP-Glo™
13ACC2ADP-Glo™
14NAD(P)H Dehydrogenase (NQO)NQO1fluorescence-based
15Nucleotide Metabolism PathwayDHODHfluorescence-based
16dDHODH (dog)fluorescence-based
17mDHODH (mouse)fluorescence-based
18rDHODH (rat)fluorescence-based

Phosphodiesterase (PDE) Assays

BellBrook labs社のTranscreener AMP2/GMP2 FP PDE assay platformを用いてPED活性を測定します。
Target name
1PDE1A
2PDE1B
3PDE1C
4PDE2A
5PDE3A
6PDE3B
7PDE4A
8PDE4B
9PDE4C
10PDE4D
11PDE4D2
12PDE5A
13PDE7A
14PDE7B
15PDE8A
16PDE9A
17PDE10A

Ubiquitin-Proteasome Pathway Assays

DUBは、ユビキチン-AMC蛍光基質を用いて活性を測定します。
Target name
1A20
2Ataxin3
3BAP1
4MYSM1
5NEDP1
6SENP1
7SENP2
8UCHL1
9UCHL3
10USP2
11USP4
12USP5
13USP7
14USP8
15USP10
16USP11
17USP13
18USP14
19USP20
20USP25
21USP28
22USP30
2320S Proteasome

Heat Shock Protein Assays

蛍光偏向(FP)アッセイによりHeat Shock Protein (HSP)酵素活性を測定します。
Target name
1HSP90a
2HSP90b

Replication-related Assays

Target nameAssay format
1MTH1 (NUDT1)PPiLight™
2MTHFD1fluorescence-based
3MTHFD2fluorescence-based