Biochemical Assay - Safety Panel Assays

Reaction Biology社およびEuroscrrenFAST (旧Euroscreen社)では、GPCR、イオンチャネル、トランスポーター、CYP等、ヒトにおいて薬物有害反応を引き起こす可能性のある幅広いターゲットに対する、化合物のオフターゲットスクリーニングに有用なパネルアッセイを提供しています。新薬開発の早い段階での安全性評価等にご利用いただけます。

• Euroscreen Safety Panel (44ターゲット)
• Reaction Biology In Vitro Evaluation of Safety and Toxicity (InVEST) panel (51ターゲット)

Safety panel (Euroscreen)

44種のGPCR、トランスポーター、イオンチャネル、各種酵素のパネルです。

	Target family	Target name	Species	Readout
1	GPCR	Adenosine A2A	Human	Radioligand Binding
2	GPCR	Adrenergic alpha1a	Human	Radioligand Binding
3	GPCR	Adrenergic alpha2a	Human	Radioligand Binding
4	GPCR	Adrenergic beta2	Human	Radioligand Binding
5	GPCR	Cannabinoid CB1	Human	Radioligand Binding
6	GPCR	Dopamine D1	Human	Radioligand Binding
7	GPCR	Dopamine D2S	Human	Radioligand Binding
8	GPCR	Opioid Sigma	Human	Radioligand Binding
9	GPCR	Opioid delta	Human	Radioligand Binding
10	GPCR	Opioid kappa	Human	Radioligand Binding
11	GPCR	Opioid mu	Human	Radioligand Binding
12	GPCR	Histamine H1	Human	Radioligand Binding
13	GPCR	Histamine H2	Human	Radioligand Binding
14	GPCR	Melatonin MT1	Human	Radioligand Binding
15	GPCR	Melatonin MT2	Human	Radioligand Binding
16	GPCR	Muscarinic Non selective	Rat	Radioligand Binding
17	GPCR	Serotonine 5-HT1A	Human	Radioligand Binding
18	GPCR	Serotonine 5-HT1B	Human	Radioligand Binding
19	GPCR	Serotonine 5-HT2A	Human	Radioligand Binding
20	GPCR	Serotonine 5-HT2B	Human	Radioligand Binding
21	GPCR	Cholecystokinin CCK1	Human	Radioligand Binding
22	GPCR	Endothelin ETA	Human	Radioligand Binding
23	GPCR	Endothelin ETB	Human	Radioligand Binding
24	GPCR	Vasopressin V1a	Human	Radioligand Binding
25	Amine Transporter	Dopamine DA	Human	Radioligand Binding
26	Amine Transporter	Norepinephrine NE	Human	Radioligand Binding
27	Amine Transporter	Serotonin 5-HT	Human	Radioligand Binding
28	Ion Channel	Ca2+ channels L-type, DHP site	Rat	Radioligand Binding
29	Ion Channel	Ca2+ channels L-type, Verapamil site	Rat	Radioligand Binding
30	Ion Channel	K+ channel hERG	Human	Radioligand Binding
31	Ion Channel	Serotonin 5-HT3	Human	Radioligand Binding
32	Ion Channel	GABA BZD (central)	Rat	Radioligand Binding
33	Ion Channel	NMDA GRIN 1	Human	Radioligand Binding
34	Ion Channel	Nicotinic alpha4beta2	Rat	Radioligand Binding
35	Protease	AChE	Electric Eel	Fluorescence
36	Cyclooxygenase	COX-2	Human	Colorimetric
37	Monoamine Oxidase	MAO-A	Human	Luminescence
38	Phosphodiesterase	PDE-3	Human	HTRF
39	Phosphodiesterase	PDE-4	Human	HTRF
40	Cytochrome P450	1A2	Human	Luminescence
41	Cytochrome P450	2C9	Human	Luminescence
42	Cytochrome P450	2D6	Human	Luminescence
43	Cytochrome P450	2E1	Human	Luminescence
44	Cytochrome P450	3A4	Human	Luminescence

In Vitro Evaluation of Safety and Toxicity (InVEST) panel

51種のGPCR、トランスポーター、イオンチャネル、CYP450、各種酵素等のパネルです。

	Target family	Target name	Species	Assay format
1	Biogenic Amine Transporter	Serotonin Transporter	Human	Radioligand Filter Binding
2	Cholinesterase	Acetylcholinesterase	Human	Enzymatic Activity
3	Cyclooxygenase	COX-1	Ovine	Enzymatic Activity
4	Cyclooxygenase	COX-2	Human	Enzymatic Activity
5	Cytochrome P450	CYP1A2	Human	Enzymatic Activity
6	Cytochrome P450	CYP2A6	Human	Enzymatic Activity
7	Cytochrome P450	CYP2B6	Human	Enzymatic Activity
8	Cytochrome P450	CYP2C19	Human	Enzymatic Activity
9	Cytochrome P450	CYP2C8	Human	Enzymatic Activity
10	Cytochrome P450	CYP2C9	Human	Enzymatic Activity
11	Cytochrome P450	CYP2D6	Human	Enzymatic Activity
12	Cytochrome P450	CYP2E1	Human	Enzymatic Activity
13	Cytochrome P450	CYP2J2	Human	Enzymatic Activity
14	Cytochrome P450	CYP3A4	Human	Enzymatic Activity
15	Cytochrome P450	CYP3A5	Human	Enzymatic Activity
16	GPCR	Adenosine A1	Human	Radioligand Filter Binding
17	GPCR	Adrenergic Alpha 1A	Human	Radioligand Filter Binding
18	GPCR	Adrenergic Alpha 2A	Human	Radioligand Filter Binding
19	GPCR	Adrenergic Beta 1	Human	Radioligand Filter Binding
20	GPCR	Adrenergic Beta 2	Human	Radioligand Filter Binding
21	GPCR	Cholecystokinin CCK1	Human	Radioligand Filter Binding
22	GPCR	Dopamine D1	Human	Radioligand Filter Binding
23	GPCR	Dopamine D2S	Human	Radioligand Filter Binding
24	GPCR	Dopamine D3	Human	Radioligand Filter Binding
25	GPCR	Histamine H1	Human	Radioligand Filter Binding
26	GPCR	Muscarinic M1	Human	Radioligand Filter Binding
27	GPCR	Muscarinic M2	Human	Radioligand Filter Binding
28	GPCR	Muscarinic M3	Human	Radioligand Filter Binding
29	GPCR	Muscarinic M4	Human	Radioligand Filter Binding
30	GPCR	Muscarinic M5	Human	Radioligand Filter Binding
31	GPCR	Opioid delta	Human	Radioligand Filter Binding
32	GPCR	Opioid kappa	Human	Radioligand Filter Binding
33	GPCR	Opioid mu	Human	Radioligand Filter Binding
34	GPCR	Serotonin 5-HT1A	Human	Radioligand Filter Binding
35	GPCR	Serotonin 5-HT1B	Human	Radioligand Filter Binding
36	Ion Channel	GABA-A	Rat	Radioligand Filter Binding
37	Ion Channel	hERG	Human	Fluorescence Polarization
38	Ion Channel	NMDA	Rat	Radioligand Filter Binding
39	Monoamine oxidase	MAO-A	Human	Enzymatic Activity
40	Monoamine oxidase	MAO-B	Human	Enzymatic Activity
41	Nuclear Receptor	Estrogen Receptor a	Human	Fluorescence Polarization
42	Nuclear Receptor	Estrogen Receptor β	Human	Fluorescence Polarization
43	Nuclear Receptor	Glucocorticoid Receptor	Human	Fluorescence Polarization
44	Nuclear Receptor	PPARg Receptor	Human	Fluorescence Polarization
45	Nuclear Receptor	Progesterone Receptor	Human	Fluorescence Polarization
46	Phosphodiesterase	PDE3A	Human	Enzymatic Activity
47	Phosphodiesterase	PDE4A	Human	Enzymatic Activity
48	Phosphodiesterase	PDE4D	Human	Enzymatic Activity
49	Protease	ACE-1	Human	Enzymatic Activity
50	Protease	Cathepsin G	Human	Enzymatic Activity
51	Protease	Thrombin Alpha	Human	Enzymatic Activity