Reaction Biologyでは、NanoBRET™ Target Engagement (TE) Intracellular Kinase Assayを提供しており、現在、wild typeとmutantのキナーゼを合わせて337種類のターゲットでアッセイが可能です。開発中のターゲットも20種類以上あります。
本アッセイでは、BRET (生物発光共鳴エネルギー転移)を利用して、生細胞内でのターゲットキナーゼに対する化合物の相互作用を評価することができます。EnzymeベースのKinase HotSpot™ Assayと組み合わせて、お客様の目的に応じた選択・利用が可能です。

  • NanoBRET™ TE Intracellular Kinase Assay (Wild Type, 266種類)
  • NanoBRET™ TE Intracellular Kinase Assay (Mutant, 71種類)
  • NanoBRET™ TE Intracellular CDK Panel Screening (CDK panel, 20種類)

NanoBRET™ TE Intracellular Kinase Assay (Wild Type)

TargetAdditional Synonyms
1AAK1AP2 associated kinase1
2ABL1ABL; JTK7; P150; c-ABL; v-ABL
3ABL2ABLL; ARG
4ADKAK
5AKT1PKB; RAC; PRKBA; RAc-ALPHA
6AKT2PRKBB; PKBBETA; RAc-BETA
7ALK1ACVRLK1; HHT; HHT2; ORW2; SKR3; ALK-1; ALK
8ALK2ACTRI; ACVRLK2; ALK2; SKR1
9ALK4ACTRIB; ACVRLK4; SKR2; ALK4
10Aurora AAurA; AIK; ARK1; AURA; BTAK; STK15; AuroraA; Aurora A
11Aurora BAurB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AuroraB; Aurora B
12Aurora CAurC; AIE2; AIK3; STK13; Aurora C; AuroraC
13AXLEC, UFO
14BLKBLK
15BMP2KBMP2 inducible kinase, BIKe
16BMPR1ASKR5, CD292, ALK3, ACVRLK3
17BMXETK; PSCTK2; PSCTK3
18BRSK1KIAA1811
19BRSK2C11orf7; PEN11B; SAD1; STK29; FLJ41362; HUSSY-12
20BTKAGMX1; AT; ATK; BPK; PSCTK1
21CAMK1CAMK1-PEN, CAMKI
22CAMK1DCKLiK, CaM-K1, CaMKID
23CAMK1GCLICKIII, VWS1, dJ272L16.1
24CAMK2ACAMKA, KIAA0968
25CAMK2DCAMKD, MGC44911, CaMKII delta
26CAMK2GCAMK, CAMK-II, CAMKG, MGC26678
27CDK1+Cyclin B1CDC2; p34
28CDK1+Cyclin E1CDC2; p34
29CDK2+Cyclin A1p33
30CDK2+Cyclin E1p33
31CDK3+Cyclin E1CDKN3
32CDK4+Cyclin D1CMM3; PSK-J3
33CDK5+CDK5R1PSSALRE
34CDK6+Cyclin D1PLSTIRE
35CDK6+Cyclin D3PLSTIRE
36CDK7CAK1, CDKN7, STK1, p39MO15
37CDK7+Cyclin HCAK1; CDKN7; STK1; p39MO15
38CDK8+Cyclin CK35
39CDK9+Cyclin KC-2k, CDC2L4, PITALRE, TAK
40CDK9+Cyclin T1C-2k; CDC2L4; PITALRE; TAK
41CDK9+Cyclin T2C-2k; CDC2L4; PITALRE; TAK
42CDK10+Cyclin L2PISSLRE
43CDK11A+Cyclin KPITSLRE
44CDK11A+Cyclin L2PITSLRE
45CDK12+Cyclin KCRKRS; CRK7
46CDK13+Cyclin KCDC2L; CDC2L5; CHED
47CDK14+Cyclin YPFTK1; PFTAIRE1
48CDK15+Cyclin YALS2CR7; PFTK2; PFTAIRE2
49CDK16+Cyclin YPCTAIRE; PCTGAIRE; PCTK1; PCTAIRE1
50CDK17+Cyclin YPCTK2; PCTAIRE2
51CDK18+Cyclin YPCTK3; PCTAIRE; PCTAIRE3
52CDK19+Cyclin CCDC2L6, CDK11
53CDK20+Cyclin HCAK-kinase p42; CCRK; CDCH; p42; PNQALRE
54CDKL1KKIALRE
55CDKL2KKIAMRE, P56
56CDKL3NKIAMRE
57CDKL5CFAP247, EIEE2
58CHEK1CHK1
59CHEK2CHK2; CDS1; HuCds1; PP1425; RAD53
60CLK1CLK/STY; CLK
61CLK2MGC61500; hCLK2
62CLK4DKFZp686A20267, CDc-like kinase 4
63COQ8BADCK4
64CSF1RFMS
65CSKCSK
66CSNK1A1LCK1-alpha1 like, CK1a1L
67CSNK1DHCKID, CK1-delta, CK1d, CK1delta
68CSNK1ECKIe, HCKIE, MGC10398, CK1-epsilon
69CSNK1G2CK1g2, CK1-gamma2, CK1gamma2
70CSNK2A1CKII, CK2A1, CK2-alpha1, CK2alpha1, CK2a
71CSNK2A2CK2a2
72DAPK2DRP-1
73DCLK3DCAMKL3, DCDC3C
74DDR1CAK; CD167; EDDR1; MCK10; NEP; DDR
75DDR2MIG20a; NTRKR3; TKT; TYRO10
76DMPKDMPK1, DM, DM1, DM1PK, DMK
77DYRK1ADYRK; DYRK1; HP86; MNB; MNBH
78DYRK1BMIRK
79DYRK2DYRK2
80EIF2AK4GCN2
81EPHA1EPH; EPHT; EPHT1
82EPHA2ECK
83EPHA3ETK, ETK1, HEKECK, HEK4, TYRO4
84EPHA4HEK8; SEK; TYRO1
85EPHA5CEK7; EHK1; HEK7
86EPHA6EHK2, EHK-2, EPA6
87EPHA7EHK3, HEK11
88EPHA8EEK; HEK3; KIAA1459
89EPHB1ELK, NET, Hek6, EPHT2
90EPHB2DRT; ERK; Hek5; EPHT3; Tyro5
91EPHB3ETK2; HEK2; TYRO6
92EPHB4HTK; MYK1; TYRO11
93ERK1ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3
94ERN1IRE1
95ERN2IRE2
96FERTYK3, c-FER, p94-FER
97FESFPS
98FGFR1H2; H3; H4; H5; CEK; FLG; FLT2; KAL2; BFGFR; C-FGR; N-SAM
99FGFR2BEK; JWS; CEK3; CFD1; ECT1; KGFR;
100FGFR3ACH, CD333, CEK2, HSFGFR3EX, JTK4
101FGFR4TKF; JTK2; MGC20292
102FGRSRC2; c-fgr; p55c-fgr
103FLT1FLT, VEGFR1, FLT1, VEGF-R1
104FLT3FLK2; STK1; CD135
105FRKPTK5, GTK, RAK
106FYNSLK, SYN, p59-Fyn
107GAKDNAJC26
108GSK3AGSK3-alpha, GSK3a
109GSK3BGSK3-beta, GSK3b
110HCKJTK9
111HIPK2PRO0593
112HIPK3ANPK, DYRK6, FIST, FIST3, PKY, YAK1
113HIPK4FLJ32818
114HUNKHUNK
115ICKLCK2, ICK, MRK
116IGF1RJTK13, IGFR, CD221
117IKBKEIKKI; IKK-i; KIAA0151; IKK-epsilon; IKKepsilon
118INSRIR
119IRAK1Pelle
120IRAK3IRAK-M
121IRAK4NY-REN-64
122ITKEMT 2; LYK 2; PSCTK2 2
123JAK2JAK2
124JAK2 (JH1 domain)JAK2
125JAK3JAKL; L-JAK; LJAK
126JNK1 (MAPK8)JNK1-alpha1, PRKM8, SAPK1
127JNK3JNK3A; PRKM10; p493F12
128KITPBT; CD117; SCFR; c-KIT
129LATS1WARTS; wts
130LATS2FLJ13161, LATS
131LCKLCK
132LIMK1LIMK1
133LIMK2LIMK2
134LRRK2PARK8; ROCO2; FLJ45829
135LTKTYK1
136LYNLYNB
137MAP2K6MAPKK6, MEK6, PRKMK6, SAPKK3
138MAP3K2MAPKKK2, MEKK2B, MEKK2
139MAP3K3MAPKKK3, MEK kinase3, MEKK3
140MAP3K4MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412
141MAP3K10MLK2; MST; MEKK10
142MAP3K11MGC17114, MLK-3, PTK1, SPRK, MLK3
143MAP3K12DLK
144MAP3K13LZK, MEKK13
145MAP3K19M3K19, RCK, YSK4
146MAP3K21KIAA1804
147MAP4K1HPK1
148MAP4K2BL44; GCK; RAB8IP
149MAP4K3GLK
150MAP4K5KHS1, GCKR, KHS, MAPKKKK5
151MAPK1ERK2; ERT1; P42MAPK; PRKM1
152MAPK4PRKM4; Erk3-relatedErk4
153MAPK6PRKM6; ERK3p97MAPKHsT17250
154MAPK9JNK2; JNK2A; JNK2ALPHA; JNK2B; PRKM9; SAPK
155MAPK11p38-beta; P38BETA2; PRKM11; SAPK2
156MAPK14p38-alpha, CSBP, CSBP1, p38alpha, p38a
157MARK2EMK1, MGC99619, PAR-1Ba, ELKL
158MARK3CTAK1, KP78, PAR1A
159MARK4KIAA1860; MARKL1
160MAST3Microtubule-associated serine/threonine-protein kinase 3, KIAA0561
161MAST4KIAA0303
162MELKKIAA0175; PK38
163MERTKMER, MER-PEN, RP38, c-Mer
164METc-MET; HGFR; RCCP2
165MKNK2MNK2
166MLK1MLK1, PRKE1, MEKK9
167MLTKMLTK, mlklak, ZAK
168MOKRAGE
169MUSKMUSK
170MYLK2KMLC; skMLCK; MLCK2
171MYLK3caMLCK, MLCK
172MYLK4Myosin light chain kinase family member 4
173NEK1KIAA1901; NY-REN-55
174NEK11FLJ23495
175NEK2NLK1; HsPK21; HsPK 21
176NEK3HSPK36; MGC29949
177NEK4NRK2; STK2
178NEK5MGC75495
179NEK6SID6-1512
180NEK9DKFZp434D0935; MGC16714; NERCC
181NIM1KNIM1
182NLKNLK
183NRKNESK
184NUAK1ARK5; KIAA0537
185NUAK2SNARK, DKFZp434J037
186PAK4p21(CDKN1A)-activated kinase 4
187PAK6PAK6, PAK5
188PAK7PAK7, PAK5, KIAA1264, MGC26232
189PHKG1phosphorylase kinase catalytic subunit gamma 1
190PIK3C3MGC61518, PI3-kinase type 3, PI3K type 3, Vps34
191PIK3CA/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
192PIK3CB/PIK3R1PI3K, PI3KBETA, PIK3C1, p110-beta
193PIK3CD/PIK3R1p110D, P110DELTA, PI3K, PI3K-delta, p110-delta
194PIKFYVEMGC40423, PIP5K, PIP5K3, ZFYVE29, FAB1, KIAA0981
195PIM3PIM3
196PIP4K2CPIP5K2C, FLJ22055
197PIP5K1BMSS4, STM7
198PKMYT1MYT1
199PKN3PRK3, PKNbeta, RP11-545E17.1
200PLK1STPK13
201PLK2SNK
202PLK3CNK, FNK, PRK
203PLK4SAK; STK18
204PRKAA1AMPK, AMPKa1
205PRKAA2AMPK; AMPKa2
206PRKACAPKA; PKACa
207PRKACBPKAcb, MGC41879, MGC9320, PKA c-beta
208PRKCEPKCE, nPKC-epsilon, PKC-epsilon
209PRKG2PKG2, cGKII, PRKGR2
210PRKXPKX1
211PTK2FAK; FADK
212PTK6BRK
213PYK2CADTK, CAKB, FADK2, FAK2, PKB, PTK, RAFTK, CAK beta
214RETPTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET51
215RIOK2FLJ11159
216RIPK1RIP
217RIPK2RICK; RIP2; CARDIAK; CARD3; CCK; GIG30; RIP-2
218ROCK1P160ROCK
219ROCK2KIAA0619, ROC2
220RONCD136; CDw136; PTK8; RON
221ROS1(1883-2347)ROS, MCF3
222RPS6KA1RSK1, HU-1, MAPKAPK1, p90Rsk
223RPS6KA2RSK3, HU-2
224RPS6KA3RSK2, HU-3, MRX19, CLS
225RPS6KA4MSK2; RSK-B
226RPS6KA6RSK4
227SBK3SGK110
228SGK1SGK
229SGK2H-SGK2, dJ138B7.2
230SIK1MSK; SNF1LK; SIK
231SIK2SIK2
232SIK3SIK3
233SLKKIAA0204, STK2
234SNRKSNF1-related kinase
235SRCc-SRC; ASV; p60-SRC; SRC1
236STK10PRO2729; LOK
237STK11LKB1
238STK16PKL12; MPSK; TSF1
239STK17BDRAK2
240STK24MST3
241STK26MASK, MST4
242STK32BYANK2
243STK33STK33
244STK36FU
245STK38NDR1
246STK38LNDR2, KIAA0965
247TBK1CDC37; HSPCA; HSPCB; IKBKG; NAK; T2K
248TECPSCTK4
249TEKVMCM; TIE-2; VMCM1; CD202B
250TGFBR2MFS2, RIIC, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, HNPCC6
251TLK1PKU-beta, KIAA0137
252TLK2MGC44450; PKU-ALPHA
253TNK1TNK1
254TRKAMTC, TRK, TRK1
255TRKBTRKB
256TTKPYT, MPS1, ESK, MPS1L1
257TXKBTKL, PSCTK5, PTK4, RLK
258TYK2JTK1
259TYK2 (JH1 domain)JTK1
260TYK2 (JH2 domain)JTK1
261TYRO3BYK, Brt, Dtk, Sky, Tif
262ULK1UNC51; Unc51.1
263ULK2ATG1B, KIAA0623, Unc51.2
264WEE1WEE1hu
265WEE2FLJ16107, WEE1B
266YES1YES; HsT441; P61-YES; C-YES, YES1

NanoBRET™ TE Intracellular Kinase Assay (Mutant)

TargetAdditional Synonyms
1ABL1 (Q252H)ABL; JTK7; P150; c-ABL; v-ABL
2ABL1 (Y253F)ABL; JTK7; P150; c-ABL; v-ABL
3ABL1 (E255K)ABL; JTK7; P150; c-ABL; v-ABL
4ABL1 (T315I)ABL; JTK7; P150; c-ABL; v-ABL
5ABL1 (F317I)ABL; JTK7; P150; c-ABL; v-ABL
6ABL1 (F317L)ABL; JTK7; P150; c-ABL; v-ABL
7ABL1 (M351T)ABL; JTK7; P150; c-ABL; v-ABL
8ABL1 (H396P)ABL; JTK7; P150; c-ABL; v-ABL
9ACVR1 (R206H)ACTRI, ACVRLK2, ALK2, SKR6
10ACVR1 (Q207D)ACTRI, ACVRLK2, ALK2, SKR4
11ACVR1 (G328V)ACTRI, ACVRLK2, ALK2, SKR2
12ACVR1 (G356D)ACTRI, ACVRLK2, ALK2, SKR3
13AKT1 (E17K)PKB, RAC, PRKBA, RAc-ALPHA
14AKT2 (E17K)PRKBB, PKBBETA, RAc-BETA
15AKT3 (E17K)PKBG, PKB gamma, PRKBG, RAC-gamma, RAC-PK-gamma, STK-2
16AKT3 (G171R)PKBG, PKB gamma, PRKBG, RAC-gamma, RAC-PK-gamma, STK-2
17BRAF (V600E)B-Raf; BRAF1; RAFB1; B-raf-1
18BTK (E41K)AGMX1, AT, ATK, BPK, PSCTK1
19BTK (P190K)AGMX1, AT, ATK, BPK, PSCTK1
20BTK (C481S)AGMX1, AT, ATK, BPK, PSCTK1
21DDR2 (N456S)MIG20a, NTRKR3, TKT, TYRO10
22FGFR3 (G697C)ACH, CD333, CEK2, HSFGFR3EX, JTK4
23FLT3 (K663Q)FLK2; STK1; CD135
24FLT3 (R834Q)FLK2; STK1; CD135
25FLT3 (D835H)FLK2; STK1; CD135
26FLT3 (D835V)FLK2; STK1; CD135
27FLT3 (D835Y)FLK2; STK1; CD135
28FLT3 (N841I)FLK2; STK1; CD135
29FYN (Y531F)SLK, SYN, p59-FYN
30JAK2 (V617F)JAK2
31KIT (V559D)PBT; CD117; SCFR; c-KIT
32KIT (V559D,T670I)C-Kit, PBT, CD117, SCFR
33KIT (V559D,V654A)C-Kit, PBT, CD117, SCFR
34KIT (L576P)PBT; CD117; SCFR; c-KIT
35KIT (D816H)PBT; CD117; SCFR; c-KIT
36KIT (D816V)C-Kit, PBT, CD117, SCFR
37KIT (A829P)C-Kit, PBT, CD117, SCFR
38LRRK2 (R1441C)PARK8, ROCO2, FLJ45829, DKFZp434H2116
39LRRK2 (G2019S)PARK8, ROCO2, FLJ45829, DKFZp434H2112
40LRRK2 (I2020T)PARK8, ROCO2, FLJ45829, DKFZp434H2114
41MAP2K1/KRAS(G12C)MEK1, MAPKK1, MKK1, PRKMK1
42MAP2K2/KRAS(G12C)MEK2, MAPKK2, MKK2, PRKMK2
43MAPK1/KRAS(G12C)ERK2; ERT1; P42MAPK; PRKM1
44MAPK3/KRAS(G12C)ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3
45MAPK14 (T106M)p38-alpha, CSBP, CSBP1, p38alpha, p38a
46MELK (T460M)KIAA0175, PK38
47MET (P991S)c-MET, HGFR, RCCP2
48MET (T992I)c-MET, HGFR, RCCP2
49MET (V1092I)c-MET, HGFR, RCCP2
50MET (T1173I)c-MET, HGFR, RCCP2
51MET (Y1230A)c-MET, HGFR, RCCP2
52MET (Y1230C)c-MET, HGFR, RCCP2
53MET (Y1230D)c-MET, HGFR, RCCP2
54MET (Y1230H)c-MET, HGFR, RCCP2
55MET (Y1235D)c-MET; HGFR; RCCP2
56MET (M1250T)c-MET; HGFR; RCCP2
57NTRK1 (G667C)MTC, TRK, TRK2
58PDGFRA (V561D)PDGFRalpha, PDGFR2, CD140a
59PIK3CA (E542K)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
60PIK3CA (E545K)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
61PIK3CA (Q546K)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
62PIK3CA (I800L)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
63PIK3CA (M1043I)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
64PIK3CA (H1047L)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
65PIK3CA (H1047R)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
66PIK3CA (H1047Y)/PIK3R1MGC142161, MGC142163, p110-alpha, PI3K, PI3K-alpha
67RET (V804L)CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
68RET (V804M)CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
69RET (M918T)CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51
70RPS6KA3 (L608F)RSK2, HU-3, MRX19, CLS
71RPS6KA3 (I416V)RSK2, HU-3, MRX19, CLS

NanoBRET™ TE Intracellular CDK Panel Screening

TargetAdditional Synonyms
1CDK1+Cyclin B1CDC2; p34
2CDK2+Cyclin E1p33
3CDK3+Cyclin E1CDKN3
4CDK4+Cyclin D1CMM3; PSK-J3
5CDK5+CDK5R1PSSALRE
6CDK6+Cyclin D1PLSTIRE
7CDK7+Cyclin HCAK1; CDKN7; STK1; p39MO15
8CDK8+Cyclin CK35
9CDK9+Cyclin KC-2k, CDC2L4, PITALRE, TAK
10CDK10+Cyclin L2PISSLRE
11CDK11A+Cyclin KPITSLRE
12CDK12+Cyclin KCRKRS; CRK7
13CDK13+Cyclin KCDC2L; CDC2L5; CHED
14CDK14+Cyclin YPFTK1; PFTAIRE1
15CDK15+Cyclin YALS2CR7; PFTK2; PFTAIRE2
16CDK16+Cyclin YPCTAIRE; PCTGAIRE; PCTK1; PCTAIRE1
17CDK17+Cyclin YPCTK2; PCTAIRE2
18CDK18+Cyclin YPCTK3; PCTAIRE; PCTAIRE3
19CDK19+Cyclin CCDC2L6, CDK11
20CDK20+Cyclin HCAK-kinase p42; CCRK; CDCH; p42; PNQALRE