PahrmaCore Labsでは、海馬皮質ニューロンやDRGニューロンを用いたマニュアルパッチクランプアッセイ、神経細胞のCaイメージングアッセイ等のニューロサイエンスアッセイの他、Cardiac safetyに関連するランゲンドルフ灌流アッセイやPurkinje fiberを用いたAction Potentialアッセイ、iPS細胞由来の心筋細胞等を用いたアッセイ等を提供しています。
| Neuroscience Assays |
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| Hippocampal，cortical neuron Species: Neonatal Rat, mouse Cell: Fresh isolated cell Assay:Manual patch clmap Experimental setting: 22℃， 3 dose n>10, 1 Hz stimulus |
| DRG exitanility Species: Rat, mouse Cell: Fresh isolated cell Assay:Manual patch clmap Experimental setting: 22℃， 3 dose n>10, 1 Hz stimulus |
| Neurons calcium imaging Species: Rat, Guiea pig Cell: Fresh isolated cell Assay: Olympus microscope & Metaflour Positive Controls: ISO 0.1 uM Experimental setting: 22℃， 3 dose n>20, 1 Hz stimulus |
| CA1 pyramidal neurons, ion channels Species: Rat, mouse Cell: Slice Assay:Manual patch clmap Experimental setting: 22℃， 4 concentrations, n=6， 24 brain slices |
| Spinal Cord neurons, ion channels Species: Rat, mouse Cell: Slice Assay:Manual patch clmap Experimental setting: 22℃， 4 concentrations, n=6， 24 brain slices |
| Cardiac Assays |
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| Langendorff isolated perfused heart Species: Guinea pig, Rat, Mouse Specimen: Isolated heart Assay: Isolated heart perfusion Positive Controls: Dofetilide Test temperature 37℃ Experimental setting: 3 concentrations n=4 |
| Isolated heart perfusion ECG & left ventricle pressure Species: Guinea pig, Rat Specimen: Isolated heart Assay: Isolated heart perfusion Positive Controls: Dofetilide Test temperature 37℃ Experimental setting: 3 concentrations n=4 |
| Purkinje Fiber action potential (AP) Species: New Zealand rabbit Specimen: Purkinje fiber Assay: Intracellular action potential Positive Controls: Dofetilide Test temperature 37℃ Experimental setup: 3 concentrations n=4, 3 stimulation frequency |
| Myocytes action potential (AP) Species: Guinea pig Specimen: Fresh isolated Myocytes Assay: Patch clamp Positive Controls: Dofetilide Test temperature 35-37℃ Experimental setup: 3 concentrations n=3, 1 stimulation frequency |
| Cardiac myocytes contractility Species: Rat, Guinea pig Cell: Ventricular / atrial myocytes Assay: IonOptix Positive Controls: ISO 0.1 uM Experimental setting: 22℃， 3 dose n>3, 1 Hz stimulus |
| Cardiac myocytes transient Ca imagine Species: Rat, Guinea pig Cell: Ventricular / atrial myocytes Assay: IonOptix Positive Controls: ISO 0.1 uM Experimental setting: 22℃， 3 dose n>3, 1 Hz stimulus |
| iPS cell screening platform |
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| hiPS MC action potential Species: Human origin Cell: iPS CM Assay: Manual patch clamp Positive Controls: Dofetilide Experimental setting: 33℃， 3 dose n=4, single stimulus |
| hiPS DRG / hiPS SN excitability & IC Species: Human origin Cell: Sensory neuron， DRG Assay: Manual patch clamp Experimental setting: 3 dose n=4 |
| Nav1.5 fast channel Species: Human origin Cell: iPS CM Assay: Manual patch clamp Positive Controls: Flecainide |
| L-type Ca channel Species: Human origin Cell: iPS CM Assay: Manual patch clamp Positive Controls: Verapamil |
| Ik channel Species: Human origin Cell: iPS CM Assay: Manual patch clamp Positive Controls: Cisapride |