Reaction BiologyのKinase HotSpot™ Assayでは、Gold StandardのRIによるダイレクトな測定をメインに行っており、再現性が高くfalse の少ないデータを提供します。現在、700種類を超えるキナーゼを対象に試験が可能です。一部、Lipid KinasesとDiacylglycerol KinasesはADP-Glo™を使用しています。

  • HotSpot™ Wild type Kinases (398キナーゼ)
  • HotSpot™ Mutant Kinases (346キナーゼ)
  • Lipid Kinases (23キナーゼ)
  • Diacylglycerol Kinases (10キナーゼ)
  • HotSpot™ Special Kinases (15キナーゼ)

Kinase HotSpot™ Assay - Wild Type Kinases

Target name
1ABL1
2ABL2/ARG
3ACK1
4AKT1
5AKT2
6AKT3
7ALK
8ALK1/ACVRL1
9ALK2/ACVR1
10ALK3/BMPR1A
11ALK4/ACVR1B
12ALK5/TGFBR1
13ALK6/BMPR1B
14ARAF
15ARK5/NUAK1
16ASK1/MAP3K5
17Aurora A
18Aurora B
19Aurora C
20AXL
21BLK
22BMPR2
23BMX/ETK
24BRAF
25BRK
26BRSK1
27BRSK2
28BTK
29CAMK1a
30CAMK1b
31CAMK1d
32CAMK1g
33CAMK2a
34CAMK2b
35CAMK2d
36CAMK2g
37CAMK4
38CAMKK1
39CAMKK2
40CDC7/DBF4
41CDK1/cyclin A
42CDK1/cyclin B
43CDK1/cyclin E
44CDK2/cyclin A1
45CDK2/cyclin A
46CDK2/cyclin E
47CDK2/cyclin E2
48CDK2/cyclin O
49CDK3/cyclin C
50CDK3/cyclin E
51CDK3/cyclin E2
52CDK4/cyclin D1
53CDK4/cyclin D2
54CDK4/cyclin D3
55CDK5/p25
56CDK5/p35
57CDK6/cyclin D1
58CDK6/cyclin D2
59CDK6/cyclin D3
60CDK7/cyclin H
61CDK9/cyclin K
62CDK9/cyclin T1
63CDK9/cyclin T2
64CDK14 (PFTK1)/cyclin Y
65CDK15/cyclin A2
66CDK15/cyclin B1
67CDK16/cyclin Y
68CDK17/cyclin Y (PCTK2)
69CDK18/cyclin Y
70CHK1
71CHK2
72CK1a1
73CK1a1L
74CK1d
75CK1epsilon
76CK1g1
77CK1g2
78CK1g3
79CK2a
80CK2a2
81c-Kit
82CLK1
83CLK2
84CLK3
85CLK4
86c-MER
87c-MET
88COT1/MAP3K8
89CSK
90c-Src
91CTK/MATK
92DAPK1
93DAPK2
94DCAMKL1
95DCAMKL2
96DCAMKL3
97DDR1
98DDR2
99DMPK
100DMPK2/CDC42BPG
101DRAK1/STK17A
102DYRK1/DYRK1A
103DYRK1B
104DYRK2
105DYRK3
106DYRK4
107EGFR
108EPHA1
109EPHA2
110EPHA3
111EPHA4
112EPHA5
113EPHA6
114EPHA7
115EPHA8
116EPHB1
117EPHB2
118EPHB3
119EPHB4
120ERBB2/HER2
121ERBB4/HER4
122ERK1/MAPK3
123ERK2/MAPK1
124ERK5/MAPK7
125ERK7/MAPK15
126ERN1/IRE1
127ERN2/IRE2
128FAK/PTK2
129FER
130FES/FPS
131FGFR1
132FGFR2
133FGFR3
134FGFR4
135FGR
136FLT1/VEGFR1
137FLT3
138FLT4/VEGFR3
139FMS
140FRK/PTK5
141FYN
142GCK/MAP4K2
143GLK/MAP4K3
144GRK1
145GRK2
146GRK3
147GRK4
148GRK5
149GRK6
150GRK7
151GSK3a
152GSK3b
153Haspin
154HCK
155HGK/MAP4K4
156HIPK1
157HIPK2
158HIPK3
159HIPK4
160HPK1/MAP4K1
161IGF1R
162IKKa/CHUK
163IKKb/IKBKB
164IKKe/IKBKE
165IR
166IRAK1
167IRAK4
168IRR/INSRR
169ITK
170JAK1
171JAK2
172JAK3
173JNK1
174JNK2
175JNK3
176KDR/VEGFR2
177KHS/MAP4K5
178LATS1
179LATS2
180LCK
181LCK2/ICK
182LIMK1
183LIMK2
184LKB1
185LOK/STK10
186LRRK2
187LYN
188LYN B
189MAK
190MAPKAPK2
191MAPKAPK3
192MAPKAPK5/PRAK
193MARK1
194MARK2/PAR-1Ba
195MARK3
196MARK4
197MAST3
198MASTL
199MEK1
200MEK2
201MEK3
202MEK5
203MEKK1
204MEKK2
205MEKK3
206MEKK6
207MELK
208MINK/MINK1
209MKK4
210MKK6
211MKK7
212MLCK/MYLK
213MLCK2/MYLK2
214MLK1/MAP3K9
215MLK2/MAP3K10
216MLK3/MAP3K11
217MLK4
218MNK1
219MNK2
220MRCKa/CDC42BPA
221MRCKb/CDC42BPB
222MSK1/RPS6KA5
223MSK2/RPS6KA4
224MSSK1/STK23
225MST1/STK4
226MST2/STK3
227MST3/STK24
228MST4
229MUSK
230MYLK3
231MYLK4
232MYO3A
233MYO3B
234NEK1
235NEK2
236NEK3
237NEK4
238NEK5
239NEK6
240NEK7
241NEK9
242NEK11
243NIM1
244NLK
245OSR1/OXSR1
246P38a/MAPK14
247P38b/MAPK11
248P38d/MAPK13
249P38g/MAPK12
250p70S6K/RPS6KB1
251p70S6Kb/RPS6KB2
252PAK1
253PAK2
254PAK3
255PAK4
256PAK5
257PAK6
258PASK
259PBK/TOPK
260PDGFRa
261PDGFRb
262PDK1/PDPK1
263PHKg1
264PHKg2
265PIM1
266PIM2
267PIM3
268PKA
269PKAcb
270PKAcg
271PKCa
272PKCb1
273PKCb2
274PKCd
275PKCepsilon
276PKCeta
277PKCg
278PKCiota
279PKCmu/PRKD1
280PKCnu/PRKD3
281PKCtheta
282PKCzeta
283PKD2/PRKD2
284PKG1a
285PKG1b
286PKG2/PRKG2
287PKN1/PRK1
288PKN2/PRK2
289PKN3/PRK3
290PLK1
291PLK2
292PLK3
293PLK4/SAK
294PRKX
295PYK2
296RAF1
297RET
298RIPK2
299RIPK4
300RIPK5
301ROCK1
302ROCK2
303RON/MST1R
304ROS/ROS1
305RSK1
306RSK2
307RSK3
308RSK4
309SBK1
310SGK1
311SGK2
312SGK3/SGKL
313SIK1
314SIK2
315SIK3
316SLK/STK2
317SNARK/NUAK2
318SNRK
319SRMS
320SRPK1
321SRPK2
322SSTK/TSSK6
323STK16
324STK21/CIT
325STK22D/TSSK1
326STK25/YSK1
327STK32A/YANK1
328STK32B/YANK2
329STK32C/YANK3
330STK33
331STK35/CLIK1
332STK38/NDR1
333STK38L/NDR2
334STK39/STLK3
335SYK
336TAK1
337TAOK1
338TAOK2
339TAOK3/JIK
340TBK1
341TEC
342TESK1
343TESK2
344TGFBR2
345TIE2/TEK
346TLK1
347TLK2
348TNIK
349TNK1
350TRKA
351TRKB
352TRKC
353TSSK2
354TSSK3/STK22C
355TSSK4
356TTBK1
357TTBK2
358TXK
359TYK1/LTK
360TYK2
361TYRO3/SKY
362ULK1
363ULK2
364ULK3
365VRK1
366VRK2
367WNK1
368WNK2
369WNK3
370YES/YES1
371YSK4/MAP3K19
372ZAK/MLTK
373ZAP70
374ZIPK/DAPK3
375AMPK(A1/B1/G1)
376AMPK(A1/B1/G2)
377AMPK(A1/B1/G3)
378AMPK(A1/B2/G1)
379AMPK (A1/B2/G2)
380AMPK (A1/B2/G3)
381AMPK(A2/B1/G1)
382AMPK (A2/B1/G2)
383AMPK (A2/B1/G3)
384AMPK(A2/B2/G1)
385AMPK(A2/B2/G2)
386AMPK(A2/B2/G3)
387DNA-PK
388EEF2K
389EIF2AK1/HRI
390EIF2AK2
391EIF2AK3/PERK
392EIF2AK4/GCN2
393mTOR/FRAP1
394PDK1/PDHK1
395PDK2/PDHK2
396PDK3/PDHK3
397PDK4/PDHK4
398TRPM7/CHAK1

Kinase HotSpot™ Assay - Mutant Kinases

Target name
1ABL1 (E255K)
2ABL1 (E255V)
3ABL1 (F317I)
4ABL1 (F317L)
5ABL1 (G250E)
6ABL1 (H396P)
7ABL1 (M351T)
8ABL1 (Q252H)
9ABL1 (T315I)
10ABL1 (V299L)
11ABL1 (Y245F)
12ABL1 (Y245F/Y412F)
13ABL1 (Y253F)
14ABL1 (Y253H)
15AKT1 (E17K)
16AKT2 (E17K)
17AKT3 (E17K)
18AKT3 (G171R)
19ALK (C1156Y)
20ALK (F1174L)
21ALK (F1174L)-EML4
22ALK (F1174L)-NPM1
23ALK (F1174S)
24ALK (G1202R)
25ALK (G1269A)
26ALK (G1269S)
27ALK (I1171N/D1203N)
28ALK (L1152R)
29ALK (L1196M)
30ALK (L1196M/G1202R)
31ALK (R1275Q)
32ALK (S1206R)
33ALK (T1151M)
34ALK (T1151-L1152insT)
35ALK-EML4 (Eex6Aex20)
36ALK-EML4 (Eex13Aex20)
37ALK-EML4 (Eex14Aex20)
38ALK-EML4 (Eex20Aex20)
39ALK-KIF5B (Kex24Aex20)
40ALK-KLC1 (Kex8Aex20)
41ALK-NPM1
42ALK-STRN (Sex3Aex20)
43ALK-TFG
44ALK-TFG (Tex4Aex20)
45ALK-TPM1
46ALK-TPM3
47ALK2 (Q207D)
48ALK2 (R206H)
49Aurora B (G160L)
50AXL (R499C)
51BRAF (d485-489/P490Y)
52BRAF (G464V)
53BRAF (G469A)
54BRAF (K601E)
55BRAF (L597V)
56BRAF (T599_V600insT)
57BRAF (V599E)
58BRAF (V600A)
59BRAF (V600D)
60BRAF (V600K)
61BRAF (R506_K507insVLR)
62BRAF-FAM131B (Fex2Bex9)
63BRAF-KIAA1549 (Kex15Bex9)
64BRAF-KIAA1549 (Kex16Bex9)
65BRAF-SRGAP3 (Sex12Bex9)
66BTK (C481S)
67BTK (E41K)
68BTK (P190K)
69BTK (T474I)
70BTK (Y485F)
71CHK2 (I157T)
72CK1a1 (E42C)
73CK1a1 (I35C)
74CK1epsilon (R178C)
75c-Kit (d557-558)
76c-Kit (A829P)
77c-Kit (d557-558/Y823D)
78c-Kit (D816E)
79c-Kit (D816F)
80c-Kit (D816H)
81c-Kit (D816I)
82c-Kit (D816V)
83c-Kit (D816Y)
84c-Kit (D820E)
85c-Kit (D820Y)
86c-Kit (N822K)
87c-Kit (T670I)
88c-Kit (V559A)
89c-Kit (V559D)
90c-Kit (V559D/V654A)
91c-Kit (V559D/T670I)
92c-Kit (V560G)
93c-Kit (V560G/D816V)
94c-Kit (V560G/N822K)
95c-Kit (V654A)
96c-Kit (Y823D)
97c-MER (A708S)
98c-MET (D1213H)
99c-MET (D1228A)
100c-MET (D1228G)
101c-MET (D1228H)
102c-MET (D1228N)
103c-MET (D1228V)
104c-MET (D1228Y)
105c-MET (Del Ex14)
106c-MET (R970C)
107c-MET (F1200I)
108c-MET (G1163R)
109c-MET (H1094L)
110c-MET (H1094Y)
111c-MET (K1244R)
112c-MET (L1195F)
113c-MET (L1195V)
114c-MET (M1250I)
115c-MET (M1250T)
116c-MET (P991S)
117c-MET (R1227K)
118c-MET (T992I)
119c-MET (T1173I)
120c-MET (V1092I)
121c-MET (Y1230A)
122c-MET (Y1230C)
123c-MET (Y1230D)
124c-MET (Y1230H)
125c-MET (Y1230S)
126c-MET (Y1235D)
127c-MET-KIF5B (Kex24Mex14)
128c-MET-TFG (Tex5Mex15)
129c-Src (T341M)
130c-SRC (Y530F)
131DDR2 (N456S)
132DDR2 (T654M)
133EGFR (A763_Y764insFHEA)
134EGFR (A763_Y764insFQEA)
135EGFR (A767_S768insTLA)
136EGFR (C775S/T790M/L858R)
137EGFR (C797A)
138EGFR (C797G/L858R)
139EGFR (C797S)
140EGFR (C797S/L858R)
141EGFR (d746)
142EGFR (d746-750)
143EGFR (d746-750/C775S/T790M/L858R)
144EGFR (d746-750/C797A)
145EGFR (d746-750/C797S)
146EGFR (d746-750/G724S)
147EGFR (d746-750/S768I)
148EGFR (d746-750/T790M)
149EGFR (d746-750/T790M/C797S)
150EGFR (d746-750/T790M/C797S/L858R)
151EGFR (d746-750/T790M/L792F)
152EGFR (d746-750/T790M/L792H)
153EGFR (d746-750/T790M/L798I)
154EGFR (d746-750/T790M/L858R)
155EGFR (d747-749)
156EGFR (d747-749/A750P)
157EGFR (d747-752/P753S)
158EGFR (d752-759)
159EGFR (D761Y)
160EGFR (D770GY)
161EGFR (D770_N771insG)
162EGFR (D770_N771insNPG)
163EGFR (D770_N771insNPG/T790M)
164EGFR (G719C)
165EGFR (G719D)
166EGFR (G719S)
167EGFR (K716A)
168EGFR (K716A/C797S/L858R)
169EGFR (K716A/T790M/C797S/L858R)
170EGFR (K716Q/L718Q)
171EGFR (K728A)
172EGFR (K728A/T790M/C797S/L858R)
173EGFR (L718Q)
174EGFR (L747S)
175EGFR (L792F)
176EGFR (L792F/L858R)
177EGFR (L792H)
178EGFR (L792H/C797S/L858R)
179EGFR (L792H/L858R)
180EGFR (L858R)
181EGFR (L861Q)
182EGFR (N771_P772insH)
183EGFR (N771_P772insN)
184EGFR (R999A)
185EGFR (S768I)
186EGFR (T790M)
187EGFR (T790M/C797G/L858R)
188EGFR (T790M/C797S)
189EGFR (T790M/C797S/L858R)
190EGFR (T790M/L858R)
191EGFR (T790M/L792F/L858R)
192EGFR (T790M/L792H/L858R)
193EGFR (T790M/L792F/C797S/L858R)
194EGFR (T790M/L792H/C797S/L858R)
195EGFR (V769_D770insGE)
196EGFR (V948R)
197ERBB2 (A775_G776insYVMA)
198ERBB2 (D769H)
199ERBB2 (D769Y)
200ERBB2 (P780_Y781insGSP)
201ERBB2 (P1170A)
202ERBB2 (R896C)
203ERBB2 (V777L)
204ERBB2 (V777_G778insCG)
205ERBB2 (V956R)
206ERN1/IRE1 (R727A)
207ERN1/IRE1 (R728A)
208ERN1/IRE1 (R727A/R728A)
209FGFR1 (V561M)
210FGFR1 (W666R)
211FGFR1OP-FGFR1
212FGFR2 (C491A)
213FGFR2 (C491F)
214FGFR2 (C491S)
215FGFR2 (C491A/V564I)
216FGFR2 (C491A/V564L)
217FGFR2 (C491S/V564L)
218FGFR2 (E565A)
219FGFR2 (E565G)
220FGFR2 (K526E)
221FGFR2 (K641R)
222FGFR2 (K659N)
223FGFR2 (M420I)
224FGFR2 (N549H)
225FGFR2 (N549K)
226FGFR2 (R612T)
227FGFR2 (V564F)
228FGFR2 (V564I)
229FGFR2 (V564L)
230FGFR3 (V555M)
231FGFR3 (G697C)
232FGFR3 (K650E)
233FGFR3 (K650M)
234FGFR3 (K650Q)
235FGFR4 (N535K)
236FGFR4 (V550E)
237FGFR4 (V550L)
238FGFR4 (V550M)
239FLT3 (D835Y)
240FLT3 (ITD)
241FLT3 (ITD)-NPOS
242FLT3 (ITD)-W51
243FLT3 (F594_R595insR)
244FLT3 (F594_R595insREY)
245FLT3 (F691L/D835Y)
246FLT3 (R595_E596insEY)
247FLT3 (Y591_V592insVDFREYEYD)
248FLT3 (Y591-V592insVDFREYEYD/D835Y)
249FLT3 (Y591-V592insVDFREYEYD/F691L)
250FYN (Y531F)
251GSK3b (C199A)
252JAK2 (V617F)
253LRRK2 (G2019S)
254LRRK2 (I2020T)
255LRRK2 (R1441C)
256MEK1 (P124L)
257MELK (T460M)
258p38a (T106M)
259PAK2 (Y443N)
260PDGFRa (D842V)
261PDGFRa (G680R)
262PDGFRa (T674I)
263PDGFRa-FIP1L1
264PDGFRa (V561D)
265PDGFRb-TPM3
266PKA (L206R)
267PKA-DNAJB1
268PKD2 (G870E)
269PKD2/PRKD2 (G848E)
270PKMzeta
271PKN1-ANXA4 (Aex2Pex13)
272PKN1-TECR (Tex1Pex10)
273RAF1 (R391W)
274RET (A883F)
275RET (E732K)
276RET (E762Q)
277RET (G691S)
278RET (G810C)
279RET (G810R)
280RET (G810S)
281RET (G810S/M918T)
282RET (L730I)
283RET (L730M)
284RET (L790F)
285RET (M918T)
286RET (R749T)
287RET (R813Q)
288RET (R912P)
289RET (S891A)
290RET (S904F)
291RET (S904A)
292RET (V738A)
293RET (V778I)
294RET (V804E)
295RET (V804L)
296RET (V804M)
297RET (V804M/G810S)
298RET (Y791F)
299RET (Y806C)
300RET (Y806H)
301RET (Y806N)
302RET-BCR
303RET-CCDC6 (PTC1)
304RET-KIF5B (Kex15Rex14)
305RET (V804L)-KIF5B
306RET (V804M)-KIF5B
307RET-PRKAR1A (PTC2)
308RET-NCOA4 (PTC3)
309ROS1 (G2032R)
310ROS1 (G2101A)
311ROS1 (G2101C)
312ROS1-GOPC
313ROS1 (L2086F)
314ROS1-TPM3
315RSK2 (I416V)
316RSK2 (L608F)
317TIE2 (A1124V)
318TIE2 (P883A)
319TIE2 (R849W)
320TIE2 (R915C)
321TIE2 (Y897C)
322TIE2 (Y897H)
323TIE2 (Y897H/R915C)
324TIE2 (Y897S)
325TIE2 (Y1108F)
326TRKA (A608D)
327TRKA (F589L)
328TRKA (G595R)
329TRKA (G595R/A608D)
330TRKA (G595R/L657M)
331TRKA (G595R/G667A)
332TRKA (G595R/G667C)
333TRKA (G595R/G667S)
334TRKA (G667C)
335TRKA (G667S)
336TRKA (L657M)
337TRKA-TFG (TRK-T3)
338TRKA-TPM3
339TRKA-TPR
340TRKC (G623E)
341TRKC (G623R)
342TRKC (G623R/L686M)
343TRKC (G696A)
344TRKC (L686M)
345YES1 (T348I)
346ZAP70 (Y319F)

Kinase Assay - Lipid Kinases

Target name
1PI3K (p110a(E542K)/p85a)
2PI3K (p110a(E545K)/p85a)
3PI3K (p110a(H1047R)/p85a)
4PI3K (p110a/p65a)
5PI3Ka (p110a/p85a)
6PI3Kb (p110b/p85a)
7PI3Kd (p110d/p85a)
8PI3Kg (p120g)
9PI3KC2a/PIK3C2A
10PI3KC2b/PIK3C2B
11PI3KC3
12PI4K2A
13PI4Ka
14PI4Kb
15PIP5K1A
16PIP5K1B
17PIP5K1C
18SPHK1
19SPHK2
20PIP4K2A/PIP5K2A
21PIP4K2B/PIP5K2B
22PIP4K2C/PIP5K2C
23PIP5K3/PYKFYVE

Kinase Assay - Diacylglycerol Kinases

Target name
1DGKA
2DGKB
3DGKD
4DGKE
5DGKG
6DGKH
7DGKI
8DGKK
9DGKQ
10DGKZ

Kinase HotSpot™ Assay - Special Kinases

Target name
1CDK8/cyclin C
2CDK12/cyclin K
3CDK12 (R722C)/cyclin K
4CDK13/cyclin K
5CDK19/cyclin C
6ERK5/MAPK7 (CD)
7GAK
8IRAK2
9JAK2 JH1&2
10NEK8
11RIPK3
12TTK
13TYK2 JH1&2
14WEE1
15WEE2